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Oxidative stress assessment in intestine of newborn rats submitted to hypoxia and reoxygenation with tadalafil
Teixeira, Luciana Cristina; Fujiki, Renato Toshio Murashita; Souza, Caio Vinicius Coitinho Paiva de; Souza, Camila Mendes de; Mismetti, Maysa Moreira; Artigiane-Neto, Ricardo; Montero, Edna Frasson de Souza; Martins, José Luiz.
Affiliation
  • Teixeira, Luciana Cristina; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. BR
  • Fujiki, Renato Toshio Murashita; UNIFESP. Sao Paulo. BR
  • Souza, Caio Vinicius Coitinho Paiva de; UNIFESP. Sao Paulo. BR
  • Souza, Camila Mendes de; UNIFESP. Sao Paulo. BR
  • Mismetti, Maysa Moreira; Faculdade de Medicina Santa Marcelina. Sao Paulo. BR
  • Artigiane-Neto, Ricardo; UNIFESP. Department of Pathology. Sao Paulo. BR
  • Montero, Edna Frasson de Souza; UNIFESP. Department of Surgery. Surgical Gastroenterology Division. Sao Paulo. BR
  • Martins, José Luiz; UNIFESP. Pediatric Surgery Division. Sao Paulo. BR
Acta cir. bras ; 34(4): e201900407, 2019. graf
Article in En | LILACS | ID: biblio-1001083
Responsible library: BR1.1
ABSTRACT
Abstract

Purpose:

To evaluate the functional and structural response of tadalafil effects in the intestinal mucosa, using an experimental model of hypoxia and reoxygenation injury in rats.

Methods:

The animals were divided into 4 groups CTL, H/R, H/R+Td and M+Td. The newborn rats allocated in groups H/R, H/R+Td and M+Td were submitted twice a day, to a gas chamber with CO2 at 100% for 10 minutes and afterward reoxygenation with O2 at 98% for 10 minutes, in the three first days of life. Tadalafil dose was given to newborn of group H/R+Td and to the pregnant rat of group M+Td. Histological analysis was made with hematoxylin-eosin technique and oxidative stress through nitrite and nitrate levels and lipid peroxidation.

Results:

The histological analysis showed a reduction of mucosa alterations in the groups that received tadalafil. In the oxidative stress evaluation, occurred an increase of NO levels and less lipidic peroxidation in the ileum segments that received tadalafil.

Conclusion:

Tadalafil provides tissue protection when administered independently to both, pregnant or newborns.
Subject(s)
Key words

Full text: 1 Index: LILACS Main subject: Oxygen / Oxidative Stress / Phosphodiesterase 5 Inhibitors / Tadalafil / Intestinal Mucosa / Hypoxia Type of study: Clinical_trials / Evaluation_studies / Prognostic_studies Limits: Animals / Female / Humans / Pregnancy Language: En Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article

Full text: 1 Index: LILACS Main subject: Oxygen / Oxidative Stress / Phosphodiesterase 5 Inhibitors / Tadalafil / Intestinal Mucosa / Hypoxia Type of study: Clinical_trials / Evaluation_studies / Prognostic_studies Limits: Animals / Female / Humans / Pregnancy Language: En Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article