Your browser doesn't support javascript.
loading
Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream
Martinez, Carlos Augusto Real; Campos, Fábio Guilherme; Kanno, Danilo Toshio; Meneses, Eli Cristiano; Matijascic, Gabrielle Maira; Goto, Eduardo Felipe Kim; Pereira, José Aires.
  • Martinez, Carlos Augusto Real; Universidade São Francisco. Postgraduate Program in Health Sciences. Bragança Paulista. BR
  • Campos, Fábio Guilherme; Universidade de São Paulo. Faculty of Medicine. Department of Gastroenterology. BR
  • Kanno, Danilo Toshio; USF. Faculty of Medicine. Division of Surgery. Bragança Paulista. BR
  • Meneses, Eli Cristiano; USF. Faculty of Pharmacology. Bragança Paulista. BR
  • Matijascic, Gabrielle Maira; USF. Faculty of Medicine. Bragança Paulista. BR
  • Goto, Eduardo Felipe Kim; USF. Faculty of Medicine. Bragança Paulista. BR
  • Pereira, José Aires; USF. Faculty of Medicine. Division of Pathology. Bragança Paulista. BR
Acta cir. bras ; 34(4): e201900406, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001085
ABSTRACT
Abstract

Purpose:

To evaluate the inflammatory reaction and measure the content of mucins, in the colonic mucosa without fecal stream submit to intervention with mesalazine.

Methods:

Twenty-four rats were submitted to a left colostomy and a distal mucous fistula and divided into two groups according to euthanasia to be performed two or four weeks. Each group was divided into two subgroups according daily application of enemas containing saline or mesalazine at 1.0 g/kg/day. Colitis was diagnosed by histological analysis and the inflammatory reaction by validated score. Acidic mucins and neutral mucins were determined with the alcian-blue and periodic acid of Schiff techniques, respectively. Sulfomucin and sialomucin were identified by high iron diamine-alcian blue technique. The tissue contents of mucins were quantified by computer-assisted image analysis. Mann-Whitney test was used to analyze the results establishing the level of significance of 5%.

Results:

Enemas with mesalazine in colonic segments without fecal stream decreased the inflammation score and increased the tissue content of all subtypes of mucins. The increase of tissue content of neutral, acid and sulfomucin was related to the time of intervention.

Conclusion:

Mesalazine enemas reduce the inflammatory process and preserve the content of mucins in colonic mucosa devoid of fecal stream.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Anti-Inflammatory Agents, Non-Steroidal / Colon / Mesalamine / Enema / Mucins Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: USF/BR / Universidade São Francisco/BR / Universidade de São Paulo/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Anti-Inflammatory Agents, Non-Steroidal / Colon / Mesalamine / Enema / Mucins Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: USF/BR / Universidade São Francisco/BR / Universidade de São Paulo/BR