Expression analysis of long non-coding RNAs in a renal ischemia-reperfusion injury model
Acta cir. bras
; 34(4): e201900403, 2019. tab, graf
Article
in En
| LILACS
| ID: biblio-1001087
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the long non-coding RNAs (lncRNAs) profile on renal ischemia reperfusion in a mouse model.Methods:
Microarray analysis was used to study the expression of misregulated lncRNA in a mouse model of renal ischemia reperfusion(I/R) with long ischemia time. Quantitative real-time PCR (qPCR) was used to verify the expression of selected lncRNAs and mRNAs.The potential functions of the lncRNA was analyzed by bioinformatics tools and databases.Results:
Kidney function was impaired in I/R group compared to the normal group. Analysis showed that a total of 2267 lncRNAs and 2341 messenger RNAs (mRNAs) were significantly expressed in I/R group (≥2.0-fold, p < 0.05).The qPCR result showed that lncRNAs and mRNAs expression were consistent with the microarray analysis. The co-expression network profile analysis based on five validated lncRNAs and 203 interacted mRNAs showed it existed a total of 208 nodes and 333 connections. The GO and KEEG pathway analysis results showed that multiple lncRNAs are involved the mechanism of I/R.Conclusion:
Multiple lncRNAs are involved in the mechanism of I/R.These analysis results will help us to further understand the mechanism of I/R and promote the new methods targeted at lncRNA to improve I/R injury.Key words
Full text:
1
Index:
LILACS
Main subject:
RNA, Messenger
/
Reperfusion Injury
/
RNA, Long Noncoding
/
Kidney
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2019
Type:
Article