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Improvement of clavulanic acid production in Streptomyces clavuligerus F613-1 by using a claR-neo reporter strategy
Qin, Ronghuo; Zhong, Chuanqing; Zong, Gongli; Fu, Jiafang; Pang, Xiuhua; Cao, Guangxiang.
Affiliation
  • Qin, Ronghuo; University of Jinan-Shandong Academy of Medical Sciences. School of Medicine and Life Sciences. Jinan. CN
  • Zhong, Chuanqing; Shandong Jianzhu University. Jinan. CN
  • Zong, Gongli; University of Jinan-Shandong Academy of Medical Sciences. School of Medicine and Life Sciences. Jinan. CN
  • Fu, Jiafang; University of Jinan-Shandong Academy of Medical Sciences. School of Medicine and Life Sciences. Jinan. CN
  • Pang, Xiuhua; Shandong University. School of Life Science. State Key Laboratory of Microbial Technology. Jinan. CN
  • Cao, Guangxiang; University of Jinan-Shandong Academy of Medical Sciences. School of Medicine and Life Sciences. Jinan. CN
Electron. j. biotechnol ; 28: 41-46, July. 2017. tab, ilus, graf
Article in En | LILACS | ID: biblio-1015839
Responsible library: CL1.1
ABSTRACT

Background:

Streptomyces clavuligerus was the producer of clavulanic acid, claR, a pathway-specific transcriptional regulator in S. clavuligerus, positively regulates clavulanic acid biosynthesis. In this study, the promoter-less kanamycin resistance gene neo was fused with claR to obtain strain NEO from S. clavuligerus F613-1. The claR-neo fusion strain NEO was mutated using physical and chemical mutagens and then screened under high concentrations of kanamycin for high-yield producers of clavulanic acid.

Results:

The reporter gene neo was fused downstream of claR and used as an indicator for expression levels of claR in strain NEO. After three rounds of continuous treatment and screening, the high-yield clavulanic acid-producing strain M3-19 was obtained. In the shaking flask model, the clavulanic acid titer of M3-19 reached 4.33 g/L, which is an increase of 33% over the titer of 3.26 g/L for the starting strains S. clavuligerus F613-1 and NEO.

Conclusions:

Our results indicate that neo can be effectively used as a reporter for the expression of late-stage biosynthetic genes when screening for high-yield strains and that this approach has strong potential for improving Streptomyces strains of industrial value.
Subject(s)
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Full text: 1 Index: LILACS Main subject: Streptomyces / Kanamycin / Clavulanic Acid Type of study: Prognostic_studies Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2017 Type: Article / Project document
Full text: 1 Index: LILACS Main subject: Streptomyces / Kanamycin / Clavulanic Acid Type of study: Prognostic_studies Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2017 Type: Article / Project document