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Application of a single-chain fragment variable (scFv) antibody for the confirmatory diagnosis of hydatid disease in non-endemic areas
Xu, Xiaobo; Zhang, Ruiqing; Chen, Xinhua.
  • Xu, Xiaobo; First Affiliated Hospital. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases. The Department of Hepatobiliary and Pancreatic Surgery. Hangzhou. CN
  • Zhang, Ruiqing; Digestive and Vascular Surgery Centre. Hepatobiliary and Hydatid Department. Urumqi. CN
  • Chen, Xinhua; First Affiliated Hospital. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases. The Department of Hepatobiliary and Pancreatic Surgery. Hangzhou. CN
Electron. j. biotechnol ; 29: 57-62, sept. 2017. ilus, graf, tab
Article in English | LILACS | ID: biblio-1017244
ABSTRACT

Background:

Hydatid disease is a serious parasitic disease threatening public health. Because of its rarity in non-endemic coastal areas, determining the nature and origin of a chronic, enlarged liver cystic mass is challenging in these regions. Under these circumstances, physicians need a confirmatory diagnostic tool beyond immunological and radiological examinations. This study investigated a novel human single-chain fragment variable (scFv) antibody for the confirmative diagnosis of 18 atypical hydatid disease cases in non-endemic coastal areas.

Results:

A scFv antibody against cystic echinococcosis was produced by genetic engineering and then applied to the immunohistochemical diagnosis of 18 cases of cystic echinococcosis presented in non-endemic coastal areas. The diagnosis of these cases by ultrasound and serum-based examinations was inconclusive. The 750 bp scFv antibody gene was expressed in COS-7 cells, and the antibody localized in the cytoplasm. The scFv antibody can detect the germinal layer and protoscolices of actively growing cysts but not of the degenerating protoscolices and has a diagnostic efficiency higher than that of single serum or ultrasound testing (P b 0.05). The combined use of scFv antibodies with serology and ultrasound diagnostics results in a diagnostic efficiency comparable to that of surgery. The scFv antibody can be used as a confirmatory test for the diagnosis of hydatid disease in non-endemic areas, providing a beneficial supplementary diagnostic method that complements traditional immune testing and ultrasonic radiology and thus helping physicians to effectively differentiate hydatid disease.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Echinococcosis / Echinococcosis, Hepatic / Single-Chain Antibodies Type of study: Diagnostic study Limits: Female / Humans / Male Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Digestive and Vascular Surgery Centre/CN / First Affiliated Hospital/CN

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Full text: Available Index: LILACS (Americas) Main subject: Echinococcosis / Echinococcosis, Hepatic / Single-Chain Antibodies Type of study: Diagnostic study Limits: Female / Humans / Male Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Digestive and Vascular Surgery Centre/CN / First Affiliated Hospital/CN