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Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
Strottmann, Daisy Maria; Zanluca, Camila; Mosimann, Ana Luiza Pamplona; Koishi, Andrea C; Auwerter, Nathalia Cavalheiro; Faoro, Helisson; Cataneo, Allan Henrique Depieri; Kuczera, Diogo; Wowk, Pryscilla Fanini; Bordignon, Juliano; Duarte dos Santos, Claudia Nunes.
  • Strottmann, Daisy Maria; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Zanluca, Camila; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Mosimann, Ana Luiza Pamplona; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Koishi, Andrea C; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Auwerter, Nathalia Cavalheiro; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Faoro, Helisson; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba. BR
  • Cataneo, Allan Henrique Depieri; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Kuczera, Diogo; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Wowk, Pryscilla Fanini; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Bordignon, Juliano; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
  • Duarte dos Santos, Claudia Nunes; Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba. BR
Mem. Inst. Oswaldo Cruz ; 114: e190150, 2019. tab, graf
Article in English | LILACS | ID: biblio-1020077
ABSTRACT
BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
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Full text: Available Index: LILACS (Americas) Main subject: Aedes / Zika Virus / Zika Virus Infection / Mice, Inbred BALB C Type of study: Prognostic study Limits: Animals / Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz-Fiocruz/BR

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Full text: Available Index: LILACS (Americas) Main subject: Aedes / Zika Virus / Zika Virus Infection / Mice, Inbred BALB C Type of study: Prognostic study Limits: Animals / Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz-Fiocruz/BR