Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types
Mem. Inst. Oswaldo Cruz
; 113(10): e180340, 2018. tab, graf
Article
in En
| LILACS
| ID: biblio-1040583
Responsible library:
BR1.1
ABSTRACT
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types.
Key words
Full text:
1
Index:
LILACS
Main subject:
DNA, Fungal
/
DNA Primers
/
Histoplasma
Type of study:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2018
Type:
Article