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Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species
Trevisoli, Lucas Eduardo; Bail, Larissa; Rodrigues, Luiza Souza; Conte, Danieli; Palmeiro, Jussara Kasuko; Dalla-Costa, Libera Maria.
Affiliation
  • Trevisoli, Lucas Eduardo; Faculdades Pequeno Príncipe. Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba. BR
  • Bail, Larissa; Universidade Estadual de Ponta Grossa. Ponta Grossa. BR
  • Rodrigues, Luiza Souza; Faculdades Pequeno Príncipe. Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba. BR
  • Conte, Danieli; Faculdades Pequeno Príncipe. Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba. BR
  • Palmeiro, Jussara Kasuko; Faculdades Pequeno Príncipe. Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba. BR
  • Dalla-Costa, Libera Maria; Faculdades Pequeno Príncipe. Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba. BR
Rev. Soc. Bras. Med. Trop ; 51(1): 85-87, Jan.-Feb. 2018. tab
Article in En | LILACS | ID: biblio-1041445
Responsible library: BR1.1
ABSTRACT
Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods. RESULTS. The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1.00). The kappa index of Vitek 2® Compact analysis was 0.85 (very good) and that of the conventional method was 0.63 (moderate). CONCLUSIONS . MALDI-TOF MS provided rapid and accurate results for the identification of CoNS (134; 100%).
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Full text: 1 Index: LILACS Main subject: Staphylococcus / Bacteriological Techniques / Coagulase / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Prognostic_studies Limits: Humans Language: En Journal: Rev. Soc. Bras. Med. Trop Journal subject: MEDICINA TROPICAL Year: 2018 Type: Article
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Full text: 1 Index: LILACS Main subject: Staphylococcus / Bacteriological Techniques / Coagulase / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Prognostic_studies Limits: Humans Language: En Journal: Rev. Soc. Bras. Med. Trop Journal subject: MEDICINA TROPICAL Year: 2018 Type: Article