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Effect of a calcium hydroxide-based intracanal medicament containing N-2-methyl pyrrolidone as a vehicle against Enterococcus faecalis biofilm
KIM, Taegun; KIM, Mi-Ah; HWANG, Yun-Chan; ROSA, Vinicius; DEL FABBRO, Massimo; MIN, Kyung-San.
Affiliation
  • KIM, Taegun; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • KIM, Mi-Ah; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • HWANG, Yun-Chan; Chonnam National University. School of Dentistry. Department of Conservative Dentistry. Gwangju. KR
  • ROSA, Vinicius; National University of Singapore. Faculty of Dentistry. SG
  • DEL FABBRO, Massimo; Università degli Studi di Milano. Dipartimento di Scienze Biomediche, Chirurgiche e Odontoiatriche. Milano. IT
  • MIN, Kyung-San; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
J. appl. oral sci ; 28: e20190516, 2020. graf
Article in En | LILACS, BBO | ID: biblio-1090775
Responsible library: BR28.1
ABSTRACT
Abstract This study investigated the effect of a calcium hydroxide (CH) paste (CleaniCal®) containing N-2-methyl pyrrolidone (NMP) as a vehicle on Enterococcus faecalis (E. faecalis) biofilms compared with other products containing saline (Calasept Plus™) or propylene glycol (PG) (Calcipex II®). Methodology Standardized bovine root canal specimens were used. The antibacterial effects were measured by colony-forming unit counting. The thickness of bacterial microcolonies and exopolysaccharides was assessed using confocal laser scanning microscopy. Morphological features of the biofilms were observed using field-emission scanning electron microscopy (FE-SEM). Bovine tooth blocks covered with nail polish were immersed into the vehicles and dispelling was observed. The data were analyzed using one-way analysis of variance and Tukey tests (p<0.05). Results CleaniCal® showed the highest antibacterial activity, followed by Calcipex II® (p<0.05). Moreover, NMP showed a higher antibacterial effect compared with PG (p<0.05). The thickness of bacteria and EPS in the CleaniCal® group was significantly lower than that of other materials tested (p<0.05). FE-SEM images showed the specimens treated with Calasept Plus™ were covered with biofilms, whereas the specimens treated with other medicaments were not. Notably, the specimen treated with CleaniCal® was cleaner than the one treated with Calcipex II®. Furthermore, the nail polish on the bovine tooth block immersed in NMP was completely dispelled. Conclusions CleaniCal® performed better than Calasept Plus™ and Calcipex II® in the removal efficacy of E. faecalis biofilms. The results suggest the effect might be due to the potent dissolving effect of NMP on organic substances.
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Full text: 1 Index: LILACS Main subject: Pyrrolidinones / Root Canal Irrigants / Calcium Hydroxide / Enterococcus faecalis / Biofilms / Anti-Bacterial Agents Type of study: Evaluation_studies Limits: Animals Language: En Journal: J. appl. oral sci Journal subject: ODONTOLOGIA Year: 2020 Type: Article

Full text: 1 Index: LILACS Main subject: Pyrrolidinones / Root Canal Irrigants / Calcium Hydroxide / Enterococcus faecalis / Biofilms / Anti-Bacterial Agents Type of study: Evaluation_studies Limits: Animals Language: En Journal: J. appl. oral sci Journal subject: ODONTOLOGIA Year: 2020 Type: Article