Evaluation of the cytotoxic and genotoxic effects by melamine and cyanuric acid co-exposure in human embryonic kidney 293 cells
Braz. j. med. biol. res
;
53(5): e9331, 2020. graf
Article
in English
| LILACS
| ID: biblio-1098113
ABSTRACT
The melamine and cyanuric acid (CA) complex has been suggested to cause the toxic effects observed in melamine-contaminated food or milk. However, the cytotoxic and genotoxic effects of co-exposure to melamine and CA are not fully clear. Therefore, the cytotoxic effects of melamine and CA were first examined by co‐exposure in human kidney 293 cells using the MTT assay. During a 24-h period for the three concentrations tested (0.5, 1, and 5 mg/mL), neither melamine nor CA alone showed significant toxic effects on 293 cells at 0.5 mg/mL, while higher concentrations led to decreased in cell viability. However, co-exposure to several combinations of melamine and CA [1001, 101, 110, and 1100 (vv), at a final concentration of 0.5 mg/mL] did cause cytotoxicity with higher levels of CA leading to higher cytotoxicity. By contrast, while neither melamine nor CA alone induced phosphorylated-H2AX (γH2AX) foci formation, melamine and CA at a 1001 ratio induced γH2AX foci 24 h post-treatment. The alkaline comet assay also revealed the presence of DNA damage following melamine and CA co-exposure. In vivo assay also revealed the presence of melamine-CA complex in the kidney. These data indicated that the cytotoxic and genotoxic effects of melamine and CA co-exposure differ from those of melamine or CA alone.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Triazines
/
DNA Damage
/
Cell Survival
/
Kidney
Limits:
Animals
/
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2020
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Hangzhou Normal University School of Medicine/CN
/
Hospital Infection Control Department, Zhejiang Provincial TCM Hospital/CN
/
Zhejiang University School of Medicine/CN
/
Zhejiang University School of Public Health/CN
/
Zhejiang-California International Nanosystems Institute/CN
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