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Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
García, Patricia; Bizama, Carolina; Rosa, Lorena; Espinoza, Jaime A; Weber, Helga; Cerda-Infante, Javier; Sánchez, Marianela; Montecinos, Viviana P; Lorenzo-Bermejo, Justo; Boekstegers, Felix; Dávila-López, Marcela; Alfaro, Francisca; Leiva-Acevedo, Claudia; Parra, Zasha; Romero, Diego; Kato, Sumie; Leal, Pamela; Lagos, Marcela; Roa, Juan Carlos.
  • García, Patricia; Pontificia Universidad Católica de Chile. Faculty of Medicine. Department of Pathology. Santiago. CL
  • Bizama, Carolina; Pontificia Universidad Católica de Chile. Faculty of Medicine. Department of Pathology. Santiago. CL
  • Rosa, Lorena; Pontificia Universidad Católica de Chile. Faculty of Medicine. Department of Pathology. Santiago. CL
  • Espinoza, Jaime A; Karolinska Institutet. Division of Genome Biology. Department of Medical Biochemistry and Biophysics. Stockholm. SE
  • Weber, Helga; Universidad de La Frontera. Scientific and Technological Bioresource Nucleus (BIOREN). Center of Excellence in Translational Medicine (CEMT). Temuco. CL
  • Cerda-Infante, Javier; Pontificia Universidad Católica de Chile. Department of Hematology Oncology; Cellular and Molecular Biology. Santiago. CL
  • Sánchez, Marianela; Pontificia Universidad Católica de Chile. Department of Hematology Oncology. Santiago. CL
  • Montecinos, Viviana P; Pontificia Universidad Católica de Chile. Department of Hematology Oncology. Santiago. CL
  • Lorenzo-Bermejo, Justo; University of Heidelberg. Institute of Medical Biometry and Informatics. Heidelberg. DE
  • Boekstegers, Felix; University of Heidelberg. Institute of Medical Biometry and Informatics. Heidelberg. DE
  • Dávila-López, Marcela; University of Gothenburg. Sahlgrenska Academy. Gothenburg. SE
  • Alfaro, Francisca; Pontificia Universidad Católica de Chile. Faculty of Medicine. Department of Pathology. Santiago. CL
  • Leiva-Acevedo, Claudia; Pontificia Universidad Católica de Chile. Faculty of Medicine. Department of Pathology. Santiago. CL
  • Parra, Zasha; Complejo Asistencial Dr. Sótero del Río. Cytogenetics Laboratory. Santiago. CL
  • Romero, Diego; Pontificia Universidad Católica de Chile. Faculty of Medicine. Department of Pathology. Santiago. CL
  • Kato, Sumie; Pontificia Universidad Católica de Chile. Faculty of Medicine. Division of Obstetrics and Gynecology. Santiago. CL
  • Leal, Pamela; Universidad de La Frontera. Scientific and Technological Bioresource Nucleus (BIOREN). Center of Excellence in Translational Medicine (CEMT). Temuco. CL
  • Lagos, Marcela; Pontificia Universidad Católica de Chile. Faculty of Medicine. Department of Clinical Laboratory. Santiago. CL
  • Roa, Juan Carlos; Pontificia Universidad Católica de Chile. Millennium Institute of Immunology and Immunotherapy. Department of Pathology. Santiago. CL
Biol. Res ; 53: 13, 2020. tab, graf
Article in English | LILACS | ID: biblio-1100919
ABSTRACT

BACKGROUND:

Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry.

RESULTS:

After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice.

CONCLUSIONS:

The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Antigens, Tumor-Associated, Carbohydrate / Indians, South American / Gallbladder Neoplasms Limits: Animals / Humans / Male Country/Region as subject: South America / Chile Language: English Journal: Biol. Res Journal subject: Biology Year: 2020 Type: Article Affiliation country: Chile / Germany / Sweden Institution/Affiliation country: Complejo Asistencial Dr. Sótero del Río/CL / Karolinska Institutet/SE / Pontificia Universidad Católica de Chile/CL / Universidad de La Frontera/CL / University of Gothenburg/SE / University of Heidelberg/DE

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Full text: Available Index: LILACS (Americas) Main subject: Antigens, Tumor-Associated, Carbohydrate / Indians, South American / Gallbladder Neoplasms Limits: Animals / Humans / Male Country/Region as subject: South America / Chile Language: English Journal: Biol. Res Journal subject: Biology Year: 2020 Type: Article Affiliation country: Chile / Germany / Sweden Institution/Affiliation country: Complejo Asistencial Dr. Sótero del Río/CL / Karolinska Institutet/SE / Pontificia Universidad Católica de Chile/CL / Universidad de La Frontera/CL / University of Gothenburg/SE / University of Heidelberg/DE