Evaluation of the Genotoxicity of Endodontic Materials for Deciduous Teeth Using the Comet Assay
Pesqui. bras. odontopediatria clín. integr
;
21: e0060, 2021. tab
Article
in English
| LILACS, BBO
| ID: biblio-1250451
ABSTRACT
ABSTRACT Objective:
To evaluate genotoxicity of zinc oxide, P. A. calcium hydroxide, mineral trioxide aggregate and an iodoform paste using comet assay on human lymphocytes. Material andMethods:
Two positive controls were used methyl-methanesulfonate for the P.A. calcium hydroxide and mineral trioxide aggregate; and doxorubicin for the iodoform paste and zinc oxide. There were also two negative controls distilled water for the P.A. calcium hydroxide and mineral trioxide aggregate; and DMSO for the iodoform paste and zinc oxide. Comets were identified using fluorescence microscopy and 100 of them were counted on each of the three slides analyzed per drug test. A damage index was established, taking into consideration the score pattern that had previously been determined from the size and intensity of the comet tail. Analysis of variance, followed by Tukey's test, was used to compare the means of the DNA damage indices.Results:
The DNA damage index observed for mineral trioxide aggregate (7.08 to 8.58) and P.A. calcium hydroxide (6.50 to 8.33), which were similar to negative control index. On the other hand, damage index for zinc oxide (104.7 to 218.50) and iodoform paste (115.7 to 210.7) were similar to positive control index.Conclusion:
Iodoform paste and zinc oxide showed genotoxicity at all concentrations used.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tooth, Deciduous
/
Zinc Oxide
/
Comet Assay
/
Genotoxicity
/
Mutagenicity Tests
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2021
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Ceará/BR
/
State University of Feira de Santana/BR
/
State University of Southwest of Bahia/BR
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