Association between interleukin-10 polymorphisms and CD4+CD25+FOXP3+ T cells in asthmatic children
J. pediatr. (Rio J.)
;
97(5): 546-551, Sept.-Oct. 2021. tab, graf
Article
in English
| LILACS
| ID: biblio-1340156
ABSTRACT
Abstract Objective:
The aim of this study was to evaluate the association between possible functional interleukin-10 (IL-10) polymorphisms, IL-10 expression and regulatory T cells (Tregs) frequency, and/or asthma severity in a sample of children and adolescents.Methods:
This is a nested case-control genetic association study. The study sample consisted of children and adolescents aged 8-14 from public schools. Four polymorphisms of the IL-10 gene (rs1518111, rs3024490, rs3024496, rs3024491) were genotyped in asthmatic subjects and controls using real-time PCR. Tregs cells and IL-10 were analyzed in peripheral blood mononuclear cells by flow cytometry. The severity of asthma was defined according to the Global Initiative for Asthma (GINA) guideline.Results:
One hundred twenty-three asthmatic subjects and fifty-eight controls participated in the study. The single nucleotide polymorphism (SNP) rs3024491 (T allele) showed association with asthma severity, presenting a higher frequency in patients in the moderate asthma group. The T allele of variant rs3024491 also showed an association with reduced IL-10 levels (p = 0.01) and with increased Tregs frequency (p = 0.01). The other variants did not present consistent associations.Conclusions:
Our results suggest that moderate asthma is associated with a higher frequency of the T allele in the SNP rs3024491. In addition, the variant rs3024491 (TT) was associated with a reduction in IL-10 production and an increased percentage of Tregs cells, suggesting possible mechanisms that influence asthma severity.
Full text:
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Index:
LILACS (Americas)
Main subject:
Asthma
/
Interleukin-10
Type of study:
Risk factors
Limits:
Adolescent
/
Child
/
Humans
Language:
English
Journal:
J. pediatr. (Rio J.)
Journal subject:
Pediatrics
Year:
2021
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)/BR
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