Fibroblast growth factor receptor 3 gene (FGFR3) mutations in high-grade muscle-invasive urothelial bladder cancer in a Brazilian population: evaluation and prevalence
Einstein (Säo Paulo)
; 20: eAO6450, 2022. tab, graf
Article
in En
| LILACS
| ID: biblio-1364794
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Objective To understand the feasibility of FGFR3 tests in the Brazilian public health context, and to sample the mutational burden of this receptor in high-grade muscle invasive bladder cancer. Methods A total of 31 patients with high-grade muscle-invasive bladder cancer were included in the present study. Either transurethral resection of bladder tumor or radical cystectomy specimens were analyzed. Formalin-fixed paraffin-embedded tissue blocks were sectioned, hematoxylin and eosin stained, and histologic sections were reviewed. Total RNA was extracted using the RNeasy DSP formalin-fixed paraffin-embedded kit. Qualitative results were displayed in Rotor-Gene AssayManager software. Results Six patients were excluded. From the samples analyzed, four (16.7%) were considered inadequate and could not have their RNA extracted. Two patients presented FGFR3 mutations, accounting for 9.5% of material available for adequate analysis. The two mutations detected included a Y373C mutation in a male patient and a S249C mutation in a female patient. Conclusion FGFR3 mutations could be analyzed in 84% of our cohort and occurred in 9.5% of patients with high-grade muscle invasive bladder cancer in this Brazilian population. FGFR3 gene mutations are targets for therapeutic drugs in muscle-invasive bladder cancer. For this reason, know the frequency of these mutations can have a significant impact on public health policies and costs provisioning.
Key words
Full text:
1
Index:
LILACS
Main subject:
Urinary Bladder Neoplasms
/
Carcinoma, Transitional Cell
/
Receptor, Fibroblast Growth Factor, Type 3
Type of study:
Prevalence_studies
/
Qualitative_research
/
Risk_factors_studies
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Einstein (Säo Paulo)
Journal subject:
MEDICINA
Year:
2022
Type:
Article