Cytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cells
Pesqui. bras. odontopediatria clín. integr
;
22: e210114, 2022. tab, graf
Article
in English
| LILACS, BBO
| ID: biblio-1365227
ABSTRACT
ABSTRACT Objective To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods The culture of hPDLSCs was established. Cell density was set at 2 × 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 14 and 116) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey's test (α=0.05). Results At 24 h, pure extract of MTA Repair HP and Biodentine 116 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 14 and 116, and White MTA 116. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Periodontal Ligament
/
Root Canal Filling Materials
/
Stem Cells
/
Cytotoxicity Tests, Immunologic
/
Dental Cements
Type of study:
Diagnostic study
/
Evaluation studies
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2022
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
University of São Paulo/BR
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