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Cytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cells
Pedrosa, Marlus da Silva; Alves, Tomaz; Rahhal, Juliana Garuba; Nogueira, Fernando Neves; Sipert, Carla Renata.
  • Pedrosa, Marlus da Silva; University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology. São Paulo. BR
  • Alves, Tomaz; University of São Paulo. School of Dentistry. Department of Stomatology. São Paulo. BR
  • Rahhal, Juliana Garuba; University of São Paulo. School of Dentistry. Department of Restorative Dentistry. São Paulo. BR
  • Nogueira, Fernando Neves; University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology. São Paulo. BR
  • Sipert, Carla Renata; University of São Paulo. School of Dentistry. Department of Restorative Dentistry. São Paulo. BR
Pesqui. bras. odontopediatria clín. integr ; 22: e210114, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1365227
ABSTRACT
ABSTRACT Objective To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods The culture of hPDLSCs was established. Cell density was set at 2 × 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 14 and 116) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey's test (α=0.05). Results At 24 h, pure extract of MTA Repair HP and Biodentine 116 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 14 and 116, and White MTA 116. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Periodontal Ligament / Root Canal Filling Materials / Stem Cells / Cytotoxicity Tests, Immunologic / Dental Cements Type of study: Diagnostic study / Evaluation studies Country/Region as subject: South America / Brazil Language: English Journal: Pesqui. bras. odontopediatria clín. integr Journal subject: Dentistry Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Periodontal Ligament / Root Canal Filling Materials / Stem Cells / Cytotoxicity Tests, Immunologic / Dental Cements Type of study: Diagnostic study / Evaluation studies Country/Region as subject: South America / Brazil Language: English Journal: Pesqui. bras. odontopediatria clín. integr Journal subject: Dentistry Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR