Liver regeneration after extensive hepatectomy in rats: effect of preoperative chemotherapy with intravenous 5-fluorouracil
Acta cir. bras
;
37(9): e370901, 2022. tab, graf, ilus
Article
in English
| LILACS, VETINDEX
| ID: biblio-1402981
ABSTRACT
Purpose:
To evaluate the effect of preoperative intravenous chemotherapy with 5-fluorouracil on liver regeneration in an experimental model of major hepatectomy in rats.Methods:
Wistar rats were divided into two groups of 20 animals each and submitted to 70% hepatectomy 24 h after intravenous injection of 5-fluorouracil 20 mg/kg (fluorouracil group, FG) or 0.9% saline (control group, CG). After hepatectomy, each group was subdivided into two subgroups of 10 animals each according to the day of sacrifice (24 h or 7 days). Liver weight during regeneration, liver regeneration rate using Kwon formula, and the immunohistochemical markers proliferating cell nuclear antigen (PCNA) and Ki-67 were used to assess liver regeneration.Results:
At early phase (24 h after hepatectomy) it was demonstrated the negative effect of 5-fluorouracil on liver regeneration when assessed by Kwon formula (p < 0.0001), PCNA analysis (p = 0.02). With regeneration process complete (7 days), it was possible to demonstrate the sustained impairment of chemotherapy with 5-fluorouracil on hepatocytes regeneration phenomenon when measured by Kwon formula (p = 0.009), PCNA analysis (p = 0.0001) and Ki-67 analysis (0.001).Conclusions:
Preoperative chemotherapy with intravenous 5-fluorouracil negatively affected the mechanisms of liver regeneration after major hepatectomy in rats.
Full text:
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Index:
LILACS (Americas)
Main subject:
Chemoprevention
/
Fluorouracil
/
Hepatectomy
/
Liver Regeneration
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Year:
2022
Type:
Article
Institution/Affiliation country:
Hospital Sugisawa/BR
/
Hospital São Vicente/BR
/
Universidade Federal do Paraná/BR
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