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Análisis fitoquímico preliminar y actividad antifúngica In vitro del extracto etanólico de las hojas de Solanum hispidum pers. Colectadas en la localidad Obraje - Perú / Preliminary phytochemical analysis and in vitro antifungal activity of the ethanolic extract of the leaves of Solanum hispidum pers. collected in the locality in Obraje - Peru
Mendoza-León, Jannelle Cyndi; Fuertes Ruitón, César Máximo; Jahuira-Arias, Martha Helena.
  • Mendoza-León, Jannelle Cyndi; Instituto Nacional de Salud. Centro Nacional de Control de Calidad. Lima. PE
  • Fuertes Ruitón, César Máximo; Universidad Nacional Mayor de San Marcos. Lima. PE
  • Jahuira-Arias, Martha Helena; Instituto Nacional de Salud. Centro Nacional de Control de Calidad. Lima. PE
Rev. peru. med. exp. salud publica ; 39(3): 321-327, jul.-sep. 2022. tab, graf
Article in Spanish | LILACS | ID: biblio-1410009
RESUMEN
RESUMEN Objetivo . Analizar y determinar la actividad antifúngica in vitro del extracto etanólico de las hojas de Solanum hispidum Pers. Materiales y métodos . Se realizó el análisis fitoquímico preliminar cualitativo mediante reacciones de color y precipitación. Se investigó la actividad antifúngica in vitro frente a Candida albicans, Aspergillus brasilensis y Trichophyton mentagrophytes usando el método de difusión en pozo de agar y el ensayo de la concentración mínima inhibitoria (CMI). Resultados . El análisis fitoquímico preliminar cualitativo mostró la presencia de compuestos fenólicos, taninos, flavonoides, esteroides, alcaloides y saponinas. La actividad antifúngica in vitro fue demostrada para todos cultivos fúngicos con halos de inhibición entre 23 a 26 mm. Los valores de la CMI fueron de 125, 250 y 125 µg/mL para C. albicans, A. brasilensis y T. mentagrophytes, respectivamente. Conclusiones. El extracto etanólico de las hojas de Solanum hispidum Pers contiene importantes metabolitos secundarios y tiene moderada actividad antifúngica.
ABSTRACT
ABSTRACT Objective. To analyze and determine the in vitro antifungical activity of the ethanolic extract of the leaves of Solanum hispidum Pers. Materials and methods. We carried out a preliminary qualitative phytochemical analysis by color and precipitation reactions. We evaluated the in vitro antifungical activity against Candida albicans, Aspergillus brasilensis and Trichophyton mentagrophytes by using the agar well diffusion method and the minimum inhibitory concentration (MIC) assay. Results. Preliminary qualitative phytochemical analysis showed the presence of phenolic compounds, tannins, flavonoids, steroids, alkaloids and saponins. In vitro antifungal activity was demonstrated for all fungal cultures with inhibition halos between 23 to 26 mm. The MIC values were 125, 250, and 125 μg/mL for C. albicans, A. brasilensis, and T. mentagrophytes, respectively. Conclusions. The ethanolic extract of the leaves of Solanum hispidum Pers. contains important secondary metabolites and has moderate antifungical activity.
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Full text: Available Index: LILACS (Americas) Main subject: Plant Extracts / Plant Leaves / Solanum / Mitosporic Fungi / Antifungal Agents Type of study: Qualitative research Country/Region as subject: South America / Peru Language: Spanish Journal: Rev. peru. med. exp. salud publica Journal subject: Public Health Year: 2022 Type: Article Affiliation country: Peru Institution/Affiliation country: Instituto Nacional de Salud/PE / Universidad Nacional Mayor de San Marcos/PE

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Full text: Available Index: LILACS (Americas) Main subject: Plant Extracts / Plant Leaves / Solanum / Mitosporic Fungi / Antifungal Agents Type of study: Qualitative research Country/Region as subject: South America / Peru Language: Spanish Journal: Rev. peru. med. exp. salud publica Journal subject: Public Health Year: 2022 Type: Article Affiliation country: Peru Institution/Affiliation country: Instituto Nacional de Salud/PE / Universidad Nacional Mayor de San Marcos/PE