Your browser doesn't support javascript.
loading
m 6A RNA Methylation Decreases Atherosclerotic Vulnerable Plaque Through Inducing T Cells
Qi, Chunmei; Li, Haoran; Yu, Yongshu; Hao, Ji; Zhang, Hao; Wang, Lele; Jin, Jingjing; Zhou, Qiang; Hu, Ya; Zhang, Chengmeng; Zhang, Qingdui.
Affiliation
  • Qi, Chunmei; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Li, Haoran; Xuzhou Medical University. Department of Cardiology. Xuzhou. CN
  • Yu, Yongshu; The Second Hospital. Xuzhou Mining Group. Department of Cardiology. Xuzhou. CN
  • Hao, Ji; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Zhang, Hao; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Wang, Lele; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Jin, Jingjing; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Zhou, Qiang; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Hu, Ya; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Zhang, Chengmeng; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
  • Zhang, Qingdui; Second Affiliated Hospital. Xuzhou Medical College. Department of Cardiology. Xuzhou. CN
Rev. bras. cir. cardiovasc ; Rev. bras. cir. cardiovasc;38(1): 124-131, Jan.-Feb. 2023. tab, graf
Article in En | LILACS-Express | LILACS | ID: biblio-1423097
Responsible library: BR1.1
ABSTRACT
ABSTRACT

Introduction:

Knockdown of fat mass and obesity-associated gene (FTO) can induce N6-methyladenosine (m 6A) ribonucleic acid (RNA) methylation. The objective of this study was to explore the effect of m 6A RNA methylation on atherosclerotic vulnerable plaque by FTO knockdown.

Methods:

A total of 50 New Zealand white rabbits were randomly divided into pure high-fat group, sham operation group, vulnerable plaque group, empty load group, and FTO knockdown group (10 rabbits/group).

Results:

Flow cytometry showed that helper T (Th) cells in the FTO knockdown group accounted for a significantly higher proportion of lymphocytes than in the vulnerable plaque group and empty load group (P<0.05). Th cells were screened by cell flow. The level of m 6A RNA methylation in the FTO knockdown group was significantly higher than in the vulnerable plaque group and empty load group (P<0.05). The levels of total cholesterol, triglyceride, and low-density lipoprotein C were higher at the 12th week than at the 1st week, but the high-density lipoprotein C level was lower at the 12th week than at the 1st week. At the 12th week, the interleukin-7 level was significantly lower in the adeno-associated virus-9 (AVV9)-FTO short hairpin RNA group than in the control and AVV9-green fluorescent protein groups (P<0.001).

Conclusion:

After successfully establishing a vascular parkinsonism rabbit model, m 6A RNA methylation can decrease Th cells and vulnerable atherosclerotic plaques.
Key words

Full text: 1 Index: LILACS Language: En Journal: Rev. bras. cir. cardiovasc Journal subject: CARDIOLOGIA / CIRURGIA GERAL Year: 2023 Type: Article / Project document

Full text: 1 Index: LILACS Language: En Journal: Rev. bras. cir. cardiovasc Journal subject: CARDIOLOGIA / CIRURGIA GERAL Year: 2023 Type: Article / Project document