β-catenin ameliorates myocardial infarction by preventing YAP-associated apoptosis
Clinics
;
78: 100189, 2023. graf
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1439905
ABSTRACT
Abstract Objective:
To explore whether the effect of β-catenin on MI and MI-induced cardiomyocyte apoptosis is YAP-dependent.Methods:
The authors established an MI rat model by ligating the anterior descending branch of the left coronary artery, and an MI cell model by treating cardiomyocytes with H2O2.Results:
β-catenin downregulation was observed in MI cardiac tissues and in H2O2-treated cardiomyocytes. Lentiviral-CTNNB1 was administered to MI rats to upregulate β-catenin expression in MI cardiac tissue. β-catenin recovery reduced the myocardial infarct area, fibrosis, and apoptotic cell death in MI rats. H2O2 treatment attenuated cell viability and induced cell death in cardiomyocytes, whereas β-catenin overexpression partially reversed these changes. Moreover, H2O2 treatment caused the deactivation of Yes-Associated Protein (YAP), as detected by increased YAP phosphorylation and reduced the nuclear localization of YAP. Upregulation of β-catenin expression reactivated YAP in H2O2-treated cardiomyocytes. Reactivation of YAP was achieved by administration of Mitochonic Acid-5 (MA-5) to H2O2-treated cardiomyocytes, and deactivation of YAP by CIL56 treatment in β-catenin-overexpressing H2O2-treated cardiomyocytes. MA-5 administration increased cell viability and repressed apoptosis in H2O2-treated cardiomyocytes, whereas CIL56 treatment counteracted the effects of β-catenin overexpression on cell survival and apoptosis.Conclusions:
The present data indicate that β-catenin and YAP are effective treatment targets for MI, blocking the apoptotic death of cardiomyocytes.
Full text:
Available
Index:
LILACS (Americas)
Type of study:
Risk factors
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2023
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
The 970th Hospital of the Joint Logistic Support Force of the Peoples Liberation Army/CN
/
Yantaishan Hospital/CN
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