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Cross-genera amplification and identification of Colpodella sp. with Cryptosporidium primers in fecal samples of zoo felids from northeast China / Amplificação cruzada de gêneros e identificação de Colpodella sp. com iniciadores de Cryptosporidium em amostras fecais de zoofilia do nordeste da China
Hussain, S; Xiuyun, L; Bukhari, S. M; Mengdhao, Z; Ahmad, S; Javid, A; Chunyu, G; Hussain, A; Ali, W; Khalid, N; Ahmad, U; Lihong, T; Zhijun, H.
Affiliation
  • Hussain, S; Northeast Forestry University. College of Wildlife and Protected Area. Harbin. CN
  • Xiuyun, L; Harbin Northern Forest Zoo. Harbin. CN
  • Bukhari, S. M; University of Veterinary and Animal Sciences. Department of Wildlife and Ecology. Lahore. PK
  • Mengdhao, Z; Northeast Forestry University. College of Wildlife and Protected Area. Harbin. CN
  • Ahmad, S; University of the Punjab. Department of Entomology. Lahore. PK
  • Javid, A; University of the Punjab. Department of Entomology. Lahore. PK
  • Chunyu, G; Harbin Northern Forest Zoo. Harbin. CN
  • Hussain, A; University of Veterinary and Animal Sciences. Department of Wildlife and Ecology. Lahore. PK
  • Ali, W; University of Veterinary and Animal Sciences. Department of Wildlife and Ecology. Lahore. PK
  • Khalid, N; University of Veterinary and Animal Sciences. Department of Wildlife and Ecology. Lahore. PK
  • Ahmad, U; University of Veterinary and Animal Sciences. Department of Wildlife and Ecology. Lahore. PK
  • Lihong, T; Northeast Forestry University. College of Wildlife and Protected Area. Harvin. CN
  • Zhijun, H; Northeast Forestry University. College of Wildlife and Protected Area. Harbin. CN
Braz. j. biol ; 83: 1-6, 2023. ilus
Article in En | LILACS, VETINDEX | ID: biblio-1468920
Responsible library: BR68.1
Localization: BR68.1
ABSTRACT
The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification of protozoan is considered a more reliable and rapid method for identification than microscopy. Among these protozoans, Cryptosporidium considered being one of the important water-borne zoonotic pathogens and a major cause of a diarrheal disease named cryptosporidiosis in humans, domestic animals, and wild animals. This study was aimed to identify Cryptosporidium in zoo felids (N= 56) belonging to different zoo of China, but accidentlly Colpodella was encountered in the zoo felids sample and phylogenetic data confirmed this unexpected amplification from fecal samples using two-step nested-PCR. Phylogenetic analysis revealed the fact about the specific primers used previously by many researchers and cross-genera amplification. We came to know that genetically sequenced amplicon gives more accurate identification of species. This study suggests more investigation on Colpodella which has been neglected previously but gains the attention of researchers after identified from humans and animals and has been known to correlate with neurological symptoms in patients.
RESUMO
Os protozoários incluem muitos patógenos humanos intracelulares. A detecção acurada desses patógenos é necessária para tratar as doenças. Na epidemiologia clínica, a identificação molecular de protozoários é considerada o método de identificação mais confiável e rápido do que a microscopia. Entre esses protozoários, o Cryptosporidium é considerado um dos importantes patógenos zoonóticos transmitidos pela água e uma das principais causas de uma doença diarreica denominada criptosporidiose em humanos, animais domésticos e selvagens. Este estudo teve como objetivo identificar Cryptosporidium em zoofelídeos (N = 56) pertencentes a diferentes zoológicos da China, mas acidentalmente Colpodella foi encontrada na amostra de zoofelídeos e os dados filogenéticos confirmaram essa amplificação inesperada de amostras fecais usando nested-PCR em duas etapas. A análise filogenética revelou o fato sobre os primers específicos usados anteriormente por muitos pesquisadores e a amplificação entre gêneros. Ficamos sabendo que o amplicon sequenciado geneticamente fornece uma identificação mais acurada das espécies. Este estudo sugere mais investigação sobre Colpodella, que foi negligenciada anteriormente, mas ganha a atenção dos pesquisadores depois de identificada em humanos e animais e é conhecida por se correlacionar com sintomas neurológicos em pacientes.
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Full text: 1 Index: LILACS Main subject: Cryptosporidium / Animals, Zoo Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Braz. j. biol Journal subject: BIOLOGIA Year: 2023 Type: Article / Project document

Full text: 1 Index: LILACS Main subject: Cryptosporidium / Animals, Zoo Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Braz. j. biol Journal subject: BIOLOGIA Year: 2023 Type: Article / Project document