The role of TNF-α and NFkß in an experimental model of intestinal carcinogenesis with 1, 2-dimethyhydrazine
Acta cir. bras
; Acta cir. bras;38: e383623, 2023. tab, graf, ilus
Article
in En
| LILACS, VETINDEX
| ID: biblio-1513537
Responsible library:
BR68.1
Localization: BR68.1
ABSTRACT
Purpose:
To analyze the potential of tumor necrosis factor-α (TNF-α) and factor nuclear kappa B (NF-κB) as colorectal cancer (CRC) biomarkers in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine (1,2-DMH).Methods:
Twenty-four male Wistar rats were divided into two groups sham and 1,2-DMH. First, 1,2-DMH (20 mg/kg/week) was administered for 15 consecutive weeks. In the 25th week, proctocolectomy was conducted. Histopathological analysis, immunohistochemistry, and gene expression of TNF-α and NF-κB were performed. Statistical analysis was performed using GraphPad Prism. The location of aberrant crypt foci (ACF) was analyzed by Kruskal-Wallis' test. For analyses with two groups with parametric data, the t-test was used; for non-parametric data, the Mann-Whitney's test was used. P < 0.05 was considered significant.Results:
The number of ACF and macroscopic lesions was significantly higher (p < 0.5) in the 1,2-DMH group compared to the sham group, and most ACF were concentrated in the distal segment of the colon. There was a statistically significant increase (p < 0.5) in protein and gene expression of TNF-α and NF-κB in the 1,2-DMH group compared to the sham group.Conclusions:
Our results provide supportive evidence that TNF-α and NF-κB pathways are strongly involved in CRC development in rats and might be used as early biomarkers of CRC pathogenesis in experimental studies.Key words
Full text:
1
Index:
LILACS
Main subject:
Biomarkers, Tumor
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Rats, Wistar
/
Carcinogenesis
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2023
Type:
Article