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Is Trimethylamine N-oxide (TMAO) Associated with NF-κB mRNA Expression in Patients with Coronary Artery Disease?
Cruz, Beatriz Da; Coutinho-Wolino, Karen Salve; Cardozo, Ludmila; Pereira, Aline DAvila; Mesquita, Claudio Tinoco; Stenvinkel, Peter; Bergman, Peter; Mafra, Denise; Stockler-Pinto, Milena Barcza.
Affiliation
  • Cruz, Beatriz Da; Universidade Federal Fluminense. Niterói. BR
  • Coutinho-Wolino, Karen Salve; Universidade Federal Fluminense. Niterói. BR
  • Cardozo, Ludmila; Universidade Federal Fluminense. Niterói. BR
  • Pereira, Aline DAvila; Universidade de Vassouras. Vassouras. BR
  • Mesquita, Claudio Tinoco; Universidade Federal Fluminense. Niterói. BR
  • Stenvinkel, Peter; Karolinska Institute. Stockholm. SE
  • Bergman, Peter; Karolinska Institute. Stockholm. SE
  • Mafra, Denise; Universidade Federal Fluminense. Niterói. BR
  • Stockler-Pinto, Milena Barcza; Universidade Federal Fluminense. Niterói. BR
Int. j. cardiovasc. sci. (Impr.) ; 37: e20230113, 2024. tab, graf
Article in En | LILACS-Express | LILACS | ID: biblio-1550292
Responsible library: BR1.1
ABSTRACT
Abstract

Background:

Trimethylamine N-oxide (TMAO), a gut microbiota metabolite, is associated with cardiovascular disease (CVD) development. TMAO can trigger an inflammatory response by inducing the nuclear factor-kappa B (NF-κB) signaling cascade and increasing the expression of pro-inflammatory cytokines, contributing to the worsening of CVD. This study aimed to evaluate the association between TMAO plasma levels and inflammation in patients with coronary artery disease (CAD).

Methods:

A cross-sectional study was carried out including 29 patients with CAD. Peripheral blood mononuclear cells (PBMC) were isolated from fasting blood samples, and NF-κB and vascular cell adhesion protein 1 (VCAM1) mRNA expression were estimated using real-time quantitative PCR. We determined TMAO plasma levels by LC-MS/MS and TNF-α by ELISA. Routine biochemical parameters were evaluated using an automatic biochemical analyzer. Correlations were estimated by Spearman or Pearson test. Statistical significance was set at the level of p < 0.05.

Results:

All patients presented TMAO levels within the normal range according to EUTox (normal range 2.83 ± 1.53 mg/L; CAD patients 0.2 [0.1 to 0.2] ng/μL). TMAO plasma levels were positively correlated with NF-κB mRNA expression (0.555; p = 0.002).

Conclusion:

TMAO plasma levels may be associated with NF-κB mRNA expression in patients with CAD and may contribute to the pathogenesis of this disease.
Key words

Full text: 1 Index: LILACS Language: En Journal: Int. j. cardiovasc. sci. (Impr.) Journal subject: CARDIOLOGIA Year: 2024 Type: Article / Project document

Full text: 1 Index: LILACS Language: En Journal: Int. j. cardiovasc. sci. (Impr.) Journal subject: CARDIOLOGIA Year: 2024 Type: Article / Project document