Bacterial culture and antibiotic sensitivity from the ocular conjunctiva of horses / Cultura e sensibilidade bacteriana da conjuntiva ocular de equinos
Ciênc. rural
; 47(6): e20160753, 2017. tab
Article
in En
| LILACS
| ID: biblio-839837
Responsible library:
BR1.1
ABSTRACT
ABSTRACT The aim of this study was to identify the conjunctival bacterial flora of healthy horses in Brasilia (Distrito Federal, Brazil), and to evaluate the antimicrobial susceptibility of the isolated strains. We examined 200 eyes of 100 healthy adult horses without any eye problems, belonging to the 1st Regiment of the Cavalry Guard (RCG) of the Brazilian Army in Brasilia. Samples were collected from the inferior conjunctival fornix of both eyes. Drug sensitivity test was performed with the antibiotics gentamicin (10µg), tobramycin (10µg), chloramphenicol (30µg) and ciprofloxacin (5µg). Of the 200 samples collected, 131 (65.5%) were considered positive for bacterial growth. A total of 208 bacterial strains belonging to 19 genera were isolated, where there was prevalence of gram-positive bacteria (65%), with Staphylococcus sp. being the species of greatest incidence. It was observed that 94, 85, 81 and 68% of the isolates were sensitive to ciprofloxacin, gentamicin, chloramphenicol and tobramycin, respectively. These results can guide the empirical selection antimicrobial therapy for infections of the ocular surface of horses, pending the identification of the etiologic agent.
RESUMO
RESUMO Objetivou-se identificar a flora bacteriana conjuntival de cavalos saudáveis, residentes de Brasília (Distrito Federal - Brasil), e avaliar a susceptibilidade antimicrobiana das cepas isoladas. Foram avaliados 200 olhos de 100 equinos hígidos, adultos, saudáveis e sem alterações oculares, provenientes do 1o Regimento de Cavalaria de Guarda (RCG) do Exército de Brasília. As amostras foram coletadas do fórnix conjuntival inferior de ambos os olhos. O teste de sensibilidade foi realizado para os antimicrobianos gentamicina (10mcg), tobramicina (10mcg), cloranfenicol (30mcg) e ciprofoxacino (5mcg). Das 200 amostras coletadas, 131 (65,5%) foram consideradas positivas para o crescimento bacteriano. Um total de 208 bactérias pertencentes a 19 gêneros foram isoladas, verificando-se prevalência de bactérias gram-positivas (65%), sendo Staphylococcus sp, a espécie de maior incidência. Foi observado que 94%, 85%, 81% e 68% das bactérias isoladas foram sensíveis ao ciprofloxacino, à gentamicina, ao cloranfenicol e à tobramicina, respectivamente. Tais resultados podem direcionar a escolha empírica da terapêutica antimicrobiana nas afecções da superfície ocular de equinos, enquanto se aguarda a identificação do agente etiológico.
Full text:
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Index:
LILACS
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Language:
En
Journal:
Ciênc. rural
Journal subject:
CIENCIA
/
SAUDE AMBIENTAL
Year:
2017
Type:
Article