Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
J. appl. oral sci
; 25(1): 10-19, Jan.-Feb. 2017. tab, graf
Article
in En
| LILACS, BBO
| ID: biblio-841166
Responsible library:
BR1.1
ABSTRACT
Abstract Objectives This study aimed to evaluate the potential of adipose-derived stem cells (ASCs) combined with a modified α-tricalcium phosphate (α-TCP) or gelatin sponge (GS) scaffolds for bone healing in a rat model. Material and Methods Bone defects were surgically created in the femur of adult SHR rats and filled with the scaffolds, empty or combined with ASCs. The results were analyzed by histology and histomorphometry on days seven, 14, 30, and 60. Results Significantly increased bone repair was observed on days seven and 60 in animals treated with α-TCP/ASCs, and on day 14 in the group treated with GS/ASCs, when compared with the groups treated with the biomaterials alone. Intense fibroplasia was observed in the group treated with GS alone, on days 14 and 30. Conclusions Our results showed that the use of ASCs combined with α-TCP or GS scaffolds resulted in increased bone repair. The higher efficacy of the α-TCP scaffold suggests osteoconductive property that results in a biological support to the cells, whereas the GS scaffold functions just as a carrier. These results confirm the potential of ASCs in accelerating bone repair in in vivo experimental rat models. These results suggest a new alternative for treating bone defects.
Key words
Full text:
1
Index:
LILACS
Main subject:
Biocompatible Materials
/
Bone Regeneration
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Calcium Phosphates
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Adipose Tissue
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Stem Cell Transplantation
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Tissue Scaffolds
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Gelatin Sponge, Absorbable
Type of study:
Evaluation_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J. appl. oral sci
Journal subject:
ODONTOLOGIA
Year:
2017
Type:
Article