Human papillomavirus type distribution and HPV16 intratype diversity in southern Brazil in women with and without cervical lesions
Mem. Inst. Oswaldo Cruz
; 112(7): 492-498, July 2017. tab
Article
in En
| LILACS
| ID: biblio-841811
Responsible library:
BR1.1
ABSTRACT
BACKGROUND Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer. OBJECTIVES This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions. METHODS HPV typing was determined by L1 gene sequencing. To identify HPV16 variants, the LCR and E6 regions were sequenced, and characteristic single nucleotide variants were identified. FINDINGS A total of 445 samples were studied, with 355 from cervical scrapes and 90 from cervical biopsies. HPV was detected in 24% and 91% of these samples, respectively. The most prevalent HPV types observed were 16 (cervical, 24%; biopsies, 57%) and 58 (cervical, 12%; biopsies, 12%). Seventy-five percent of the HPV16-positive samples were classified into lineages, with 88% defined as lineage A, 10% as lineage D, and 2% as lineage B. MAIN CONCLUSIONS This study identified a high frequency of European and North American HPV16 lineages, consistent with the genetic background of the human population in southern Brazil.
Key words
Full text:
1
Index:
LILACS
Main subject:
Genetic Variation
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DNA, Viral
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Uterine Cervical Neoplasms
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Papillomavirus Infections
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Human papillomavirus 16
Type of study:
Observational_studies
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Prevalence_studies
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Prognostic_studies
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Risk_factors_studies
Limits:
Adult
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Female
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Humans
Country/Region as subject:
America do sul
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Brasil
Language:
En
Journal:
Mem Inst Oswaldo Cruz
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Mem. Inst. Oswaldo Cruz
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Mem. Inst. Oswaldo Cruz (Online)
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Memórias do Instituto Oswaldo Cruz (Impresso)
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Proceedings of the Instituto Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
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PARASITOLOGIA
Year:
2017
Type:
Article
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Project document