Effect of laser therapy on laryngeal carcinoma cell proliferation(H Ep-2)
Pesqui. bras. odontopediatria clín. integr
; 14(4): 275-282, out. 2014. tab, ilus
Article
in En
| LILACS, BBO
| ID: biblio-869251
Responsible library:
BR1264.1
ABSTRACT
Objective:
to evaluate the effect of 660nm and 780 nm laser therapy, with dose of 6.3 J/cm² and 25 mW power, either associated with nutritional stress or not, on laryngeal epidermoid carcinoma cell proliferation (H.Ep. 2). Material andMethods:
the H.Ep.2 cells were placed in a culture flask and frozen in fetal bovine serum (FBS) at -80°C, with different concentrations of the medium 5% and 10%. Laser therapy was started 24 h after cell subculturing and performed at time intervals of 6, 12, 24, 48 and 72 hours after the first irradiation, in a period of five days. After this, cell viability was verified using the MTT method. Means and standard deviation were obtained, and the Student's-t and F (ANOVA) statistical tests were used, with Tukey or Tamanhe comparisons.Results:
The group subjected to 780 nm laser showed significant differences (p 0.05). The time influenced the growth of all cultures.Conclusion:
low level laser interfered in cell proliferation and this effect could be determined by the wavelength used and the nutritional status of the cells.Key words
Full text:
1
Index:
LILACS
Main subject:
Carcinoma, Squamous Cell
/
Laryngeal Neoplasms
/
Low-Level Light Therapy
/
Cell Proliferation
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Brazilian Research in Pediatric Dentistry and Integrated Clinic
/
Pesqui. bras. odontopediatria clín. integr
/
Pesquisa brasileira em odontopediatria e clínica integrada (Impresso)
/
Pesquisa brasileira em odontopediatria e clínica integrada (Online)
Journal subject:
ODONTOLOGIA
Year:
2014
Type:
Article