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Evaluation of enemas containing sucralfate in tissue content of muc-2 protein in experimental model of diversion colitis / Avaliação de enemas com sucralfato no conteúdo tecidual da proteina muc-2 em colite de exclusão experimental
Fernandez, Oscar Orlando Araya; Pereira, José Aires; Campos, Fábio Guilherme; Araya, Carolina Mardegan; Marinho, Gabriele Escocia; Novo, Rafaela de Souza; Oliveira, Thais Silva de; Franceschi, Yara Tinoco; Martinez, Carlos Augusto Real.
Affiliation
  • Fernandez, Oscar Orlando Araya; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Pereira, José Aires; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Campos, Fábio Guilherme; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Araya, Carolina Mardegan; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Marinho, Gabriele Escocia; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Novo, Rafaela de Souza; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Oliveira, Thais Silva de; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Franceschi, Yara Tinoco; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
  • Martinez, Carlos Augusto Real; São Francisco University. Post-Graduation Program in Health Sciences. Laboratory of Medical Research. Bragança Paulista. BR
ABCD (São Paulo, Impr.) ; 30(2): 132-138, Apr.-June 2017. tab, graf
Article in En | LILACS | ID: biblio-885712
Responsible library: BR1.1
ABSTRACT
ABSTRACT

Background:

The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis.

Aim:

To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention.

Methods:

Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry).

Results:

The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01).

Conclusion:

The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.
RESUMO
RESUMO Racional Os efeitos da aplicação tópica de sucralfato (SCF) no conteúdo tecidual da proteína mucina-2 (MUC-2) ainda não foram avaliados em modelos experimentais de colite de exclusão.

Objetivo:

Mensurar o conteúdo tecidual da proteína MUC-2 na mucosa cólica sem trânsito intestinal submetida à intervenção com SCF.

Método:

Trinta e seis ratos foram submetidos à derivação intestinal por colostomia proximal terminal e fístula mucosa distal. Foram divididos em três grupos segundo recebessem clisteres contendo solução fisiológica (SF), SCF 1 g/kg/dia e SCF 2 g/kg/dia. Cada grupo foi dividido em dois subgrupos, segundo a eutanásia ser realizada após duas ou quatro semanas. O diagnóstico de colite foi estabelecido por estudo histopatológico e a intensidade inflamatória foi avaliada por escala validada. A expressão tecidual da MUC-2 foi identificada por imunoistoquímica e seu conteúdo mensurado por morfometria computadorizada.

Resultados:

A aplicação de clisteres com SCF na concentração de 2 g/kg/dia reduziu a intensidade inflamatória no cólon sem trânsito fecal. O conteúdo tecidual de MUC-2 no cólon sem trânsito dos animais submetidos à intervenção com SCF, independente do tempo de intervenção e da concentração utilizada, foi maior quando comparado aos animais tratados com SF (p<0,01). O conteúdo de MUC-2 após a intervenção com SCF na concentração de 2 g/kg/dia foi maior quando comparado aos animais submetidos à intervenção com concentração menor (p<0,01). O conteúdo de MUC-2 foi maior após intervenção com SCF na concentração de 2 g/kg/dia por quatro semanas (p<0,01).

Conclusão:

A aplicação preventiva de clisteres com SCF reduz o grau de inflamação e preserva o conteúdo tecidual de MUC-2, em segmentos desprovidos de trânsito intestinal, mostrando-se uma estratégia terapêutica válida para preservar a camada de muco que recobre o epitélio intestinal.
Subject(s)
Key words

Full text: 1 Index: LILACS Main subject: Sucralfate / Colitis / Colon / Enema / Mucin-2 / Intestinal Mucosa Type of study: Prognostic_studies Limits: Animals Language: En Journal: ABCD (São Paulo, Impr.) Journal subject: CIRURGIA GERAL / GASTROENTEROLOGIA / Procedimentos Cir£rgicos Operat¢rios / Sistema Digest¢rio Year: 2017 Type: Article

Full text: 1 Index: LILACS Main subject: Sucralfate / Colitis / Colon / Enema / Mucin-2 / Intestinal Mucosa Type of study: Prognostic_studies Limits: Animals Language: En Journal: ABCD (São Paulo, Impr.) Journal subject: CIRURGIA GERAL / GASTROENTEROLOGIA / Procedimentos Cir£rgicos Operat¢rios / Sistema Digest¢rio Year: 2017 Type: Article