Effect of melatonin on antioxidant capacity, inflammation and apoptotic cell death in lung tissue of diabetic rats
Acta cir. bras
; 33(4): 375-385, Apr. 2018. tab, graf
Article
in En
| LILACS
| ID: biblio-886280
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the effects of melatonin on antioxidant capacity, inflammation and apoptotic cell death (through expression of cleaved-caspase 3) in lung tissue samples of diabetic rats.Methods:
Thirty male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control group) was made up of healthy rats. Group 2 (diabetes group) received streptozotocin at a dose of 50 mg/kg/day for 5 days.Group 3 (diabetes plus melatonin group) received streptozotocin at a dose of 50 mg/kg/day for 5 days and then they received melatonin at a dose of 20 mg/kg/day between 28thand 35thdays of the study.Results:
Tissue MDA and MPO levels were found to be significantly higher in diabetes group compared to control group (p<0.05) whilst administration of melatonin was found to significantly lower this increase down to normal levels (p<0.05). Bronchus associated lymphoid tissue (BALT) was more severe in diabetics whereas administration of melatonin alleviated this hyperplasia. Cleaved caspase 3 activity was severe in hyperplastic BALT in diabetic rats however in lowered down to moderate level when melatonin was administered.Conclusion:
The melatonin caused an increase in antioxidant capacity and decreased the expression of cleaved-caspase 3.Key words
Full text:
1
Index:
LILACS
Main subject:
Diabetes Mellitus, Experimental
/
Caspase 3
/
Pyroptosis
/
Lung
/
Melatonin
/
Antioxidants
Type of study:
Evaluation_studies
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2018
Type:
Article