Effects of drying and storage conditions on the stability of TSH in blood spots
Arch. endocrinol. metab. (Online)
; 62(2): 201-204, Mar.-Apr. 2018. tab, graf
Article
in En
| LILACS
| ID: biblio-887641
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Objective To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening. Subjects and methods Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage. Results The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer. Conclusions Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account.
Key words
Full text:
1
Index:
LILACS
Main subject:
Thyrotropin
/
Blood Specimen Collection
/
Neonatal Screening
/
Freeze Drying
Type of study:
Evaluation_studies
Limits:
Adult
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Aged
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Female
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Humans
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Male
/
Newborn
Language:
En
Journal:
Arch. endocrinol. metab. (Online)
Journal subject:
ENDOCRINOLOGIA
/
METABOLISMO
Year:
2018
Type:
Article