Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis
Braz. j. microbiol
; 48(4): 774-781, Oct.-Dec. 2017. tab, graf
Article
in En
| LILACS
| ID: biblio-889161
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An indirect ELISA system was established for the detection of Salmonella Enteritidis. Our results confirmed that the recombinant flagellin can be one of the excellent indicators for the detection of Salmonella Enteritidis.
Key words
Full text:
1
Index:
LILACS
Main subject:
Salmonella enteritidis
/
Enzyme-Linked Immunosorbent Assay
/
Flagellin
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2017
Type:
Article