miR-483-5p promotes prostate cancer cell proliferation and invasion by targeting RBM5
Int. braz. j. urol
; 43(6): 1060-1067, Nov.-Dec. 2017. graf
Article
in En
| LILACS
| ID: biblio-892928
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Objective:
miR-483-5p has been identified as a miRNA oncogene in certain cancers. However, its role in prostate cancer has not been sufficiently investigated. In this study, we investigated the role of miR-483-5p in prostate cancer and examined RBM5 regulation by miR-483-5p. Material andmethods:
Expression levels of miR-483-5p were determined by quantitative real-time PCR. The effect of miR-483-5p on proliferation was evaluated by MTT assay, cell invasion was evaluated by trans-well invasion assays, and target protein expression was determined by western blotting in LNCaP, DU-145, and PC-3 cells. Luciferase reporter plasmids were constructed to confirm the action of miR-483-5p on downstream target gene RBM5 in HEK-293T cells.Results:
we observed that miR-483-5p was upregulated in prostate cancer cell lines and tissues. A miR-483-5p inhibitor inhibited prostate cancer cell growth and invasion in DU-145 and PC-3 cells. miR-483-5p directly bound to the 3' untranslated region (3'UTR) of RBM5 in HEK-293T cells. RBM5 overexpression inhibited prostate cancer cell growth and invasion in LNCaP cells. Enforced RBM5 expression alleviated miR-483-5p promotion of prostate cancer cell growth and invasion in LNCaP cells.Conclusion:
The present study describes a potential mechanism underlying a miR-483-5p/RBM5 link that contributes to prostate cancer development.Key words
Full text:
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Index:
LILACS
Main subject:
Prostatic Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Cell Cycle Proteins
/
Untranslated Regions
/
Tumor Suppressor Proteins
/
MicroRNAs
/
Cell Proliferation
/
DNA-Binding Proteins
/
Real-Time Polymerase Chain Reaction
Limits:
Humans
/
Male
Language:
En
Journal:
Int. braz. j. urol
Journal subject:
UROLOGIA
Year:
2017
Type:
Article