Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus
Biol. Res
; 48: 1-9, 2015. ilus, graf, tab
Article
in En
| LILACS
| ID: biblio-950776
Responsible library:
CL1.1
ABSTRACT
BACKGROUND:
In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA).RESULTS:
The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 µg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5α-cholestane-3ß,4ß,6α,7α,8ß,15α,16ß,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 µM), and for (25S) 5α-cholestane-3ß,6α,8ß,15α,16ß,26-hexol (1) and (25S)5α-cholestane-3ß,6α,7α,8ß,15α,16ß,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and and 1.02 ± 0.01 µM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production.CONCLUSION:
This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.Key words
Full text:
1
Index:
LILACS
Main subject:
Starfish
/
Dendritic Cells
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Interleukin-12 Subunit p40
/
Anti-Inflammatory Agents
Type of study:
Prognostic_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Biol. Res
Journal subject:
BIOLOGIA
Year:
2015
Type:
Article