The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
Int. braz. j. urol
;
44(3): 608-616, May-June 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-954055
ABSTRACT
ABSTRACT Purpose: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. Materials and Methods: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was induced on the right kidney at gestational day 11. The same procedure was performed in eight non-pregnant rats as control group. Three months after delivery, right ne- phrectomy was performed in order to evaluate the injured kidney. The fresh perfused kidneys were stained with anti-GFP antibody. Polymerase chain reaction (PCR) assay was also performed for the Y chromosome detection. Cell culture was performed to detect the GFP-positive cells. Technetium-99m-DMSA renal scan and single-photon emission computed tomography (SPECT) were performed after renal damage induction and 3 months later to evaluate the improvement of renal integrity. Results: The presence of FSCs was confirmed by immune histochemical staining as well as immunofluorescent imaging of the damaged part. Gradient PCR of female rat purified DNA demonstrated the presence of Y-chromosome in the damaged maternal kidney. Moreover, the culture of kidney cells showed GPF- positive cells by immuno- fluorescence microscopy. The acute renal scar was repaired and the integrity of dam- aged kidney reached to near normal levels in experimental group as shown in DMSA scan. However, no significant improvement was observed in control group. Conclusion: FSC seems to be the main mechanism in repairing of the maternal renal injury during pregnancy as indicated by Y chromosome and GFP-positive cells in the sub-cultured medium.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Wound Healing
/
Chimerism
/
Fetal Stem Cells
/
Kidney Diseases
/
Maternal-Fetal Exchange
Type of study:
Prognostic study
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2018
Type:
Article
Affiliation country:
Iran
Institution/Affiliation country:
Tehran University of Medical Sciences/IR
Similar
MEDLINE
...
LILACS
LIS