Early Detection of Bacillus anthracis From Saliva in Anticipation of a Bioterrorism Attack
Pesqui. bras. odontopediatria clín. integr
; 19(1): 4873, 01 Fevereiro 2019. ilus, tab
Article
in En
| LILACS, BBO
| ID: biblio-998253
Responsible library:
BR1264.1
ABSTRACT
Objective:
To assess potential for early detection of oral infection by B. anthracis spores for preparedness of a bioterrorism attack. Material andMethods:
The laboratory study used saliva with a range of initial anthrax concentrations, to compare detection by direct observation from conventional blood agar culture and by anthrax-specific PCR after a shorter culture in BHI broth. Three types of saliva were collected stimulated saliva, unstimulated/whole saliva, and unstimulated/whole saliva with antibiotic treatment (for negative control). Using bivariate Kruskal-Wallis and Mann-Whitney tests for statistical analysis for factors that could affecting anthrax detection, significant differences between the test groups was assumed at p<0.05.Results:
From unstimulated whole saliva heat shock treated at 62.50C, B. anthracis growth was detected with both methods. PCR detection from a BHI broth culture could shorten the time to diagnosis in comparison to conventional culture in blood agar.Conclusion:
Saliva can provide useful samples for diagnosis of oropharyngeal anthrax. In comparison to conventional culture on blood agar, shorter-term culture in BHI broth provides potential for earlier detection and diagnosis.Key words
Full text:
1
Index:
LILACS
Main subject:
Saliva
/
Bioterrorism
/
Early Diagnosis
/
Anthrax
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Screening_studies
Country/Region as subject:
Asia
Language:
En
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
ODONTOLOGIA
Year:
2019
Type:
Article