in vitro assay to evaluate the immunomodulatory effects of unrestricted somatic stem cells

ABSTRACT

Unrestricted somatic stem cells [USSC] are cord blood stem cells that have been considered as candidates for the regulation of immune responses. Therefore, potential exists for their use in the suppression of immune response after transplantation surgery. The aim of this study was evaluation of the effect of USSC on mixed lymphocyte reaction [MLR] as a model for graft rejection. USSC and mesanchymal stem cells [MSC] were isolated and cultured from cord blood and bone morrow, respectively. The immunophenotypes of USSC and MSC were evaluated by flow cytometery and USSC and MSC were cocultured with peripheral blood lymphocytes [PBL] in an MLR to evaluate the immunomodulatory effect of these cells as a percentage of the control response. Current study demonstrated that proliferation of lymphocytes in the MLR was decreased after treatment with USSC, in a similar fashion to that seen with MSC. It can be concluded that USSC have similar regulatory effects as MSC on the MLR, which can be used as an indicator for potential organ rejection after transplantation. Therefore, the immunoregulatory effect of these cells could be used in the clinic during organ transplantation and in the management of autoimmunity