Antifungal activities of some plant extracts against Fusarium solani and their molecular characterization

ABSTRACT

The present investigation was carried of the antifungal properties of leaf extract of Aegle marmelos [L.], Trianthema decandra Linn., Sphaeranthus indicus Roxb., Borreria hispida and Leucas aspera against ten clinical isolates of Fusarium solani strains from Aravind Eye Hospital, Coimbatore. The antifungal assay was determined by disc diffusion method by using the solvents such as ethanol, benzene, dimethyl formaldehyde and distilled water used, the leaves of Aegle marmelos Correa, Trianthema decandra and borreria hispida had been extracted with DMF instead of benzene. The results revealed that the ethanolic extract having significant inhibitory activity when compared to other solvent. There was no activity found in aqueous extracts against all strains. Fusarium solani [4th strain] was not inhibited by solvent extracts of all plant leaves. Based on the high inhibitory activity, the six strains were selected and the DNA were isolated from that Fusarium solani