[Quantification of 4-hydroxyisoleucine content in treated and untreated fenugreek seeds using GC]
Journal of Medicinal Plants. 2010; 9 (34): 90-94
in En
| IMEMR
| ID: emr-117707
Responsible library:
EMRO
ABSTRACT
There are some reports regarding antidiabetic activity of 4-hydroxyisoleucine, however its hypoglycemic property must be proven completely before presentating as a new drug. Preparing suitable amount of 4-hydroxyisoleucine is nesessary for supporting in vivo studies. The purpose of the present study was isolation of 4-hydroxyisoleucine, comparing 4-hydroxyisoleucine content in two samples of Fenugreek seeds collected form two distinct parts of Iran and finally the effect of germination and supplementation by isoleucine on 4-hydroxyisoleucine yield. 4-hydroxyisoleucine was successfully isolated passing seed amino acid extract through ion exchange and silica gel column and then crystallization on methanol. 4- hydroxyisoleucine content in various samples was assessed by GC. Seeds from Northwestern of Iran had higher amount of 4-hydroxyisoleucine in comparison to Central Iran. Furthermore yields from germinating seeds were 2 folds higher than the dried seeds, and could be increased 6 fold by inclusion of isoleucine supplement in the irrigation water. simple procedures can be engaged for improving yields of 4-hydroxyisoleucine isolated from fenugreek up to six-fold by using of germinated seeds supplemented with isoleucine
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Index:
IMEMR
Main subject:
Isoleucine
/
Phytotherapy
Language:
En
Journal:
J. Med. Plants
Year:
2010