Studies on Agrobacterium rhizogenes isolated from the nodules of some leguminous plants

ABSTRACT

To compare the isolates of Agrobacterium of live different Leguminous plants viz., pisum sativum, Sesbania rostrata, vigna mungo, V. radiate and V. ungiculata. Bacteria were isolated from the nodules of host species as per the standard methods. The isolated bacteria were characterized and identified as Agrobacterium rhizogenes and confirmed by Pathogenicity test. Biochemical characteristics such as exopolysaccharide, glycogen, total protein, total free amino acids and total lipid were quantified from the agrobacterial isolates. Similarly protein profile was also carried out by SDS-PAGE. From the above results it is concluded that the agrobacteria of different leguminous plants showed lot of variants in their biochemical contents and hence be treated as separate strains of A. rhizogenes