Modeling breast acini in tissue culture for detection of malignant phenotype reversion to non-malignant phenotype
IBJ-Iranian Biomedical Journal. 2009; 13 (4): 191-198
in En
| IMEMR
| ID: emr-134588
Responsible library:
EMRO
ABSTRACT
Evidence is accumulating to support disruption of tissue architecture as a powerful event in tumor formation. For the past four decades, intensive cancer research with the premise of "cancer as a cell based-disease" focused on finding oncogenes or tumor suppressor genes. However, the role of the tissue architecture was neglected. Three dimensional [3D] cell cultures which can recapitulate major aspects of the microenvironment are appropriate models for exploring cancer. For the first time in Iran, we have launched Matrigel based non-malignant, tumorigenic and reverted breast 3D cell cultures. Non-tumorigenic MCF-10A and tumorigenic MCF-7 breast cell lines were cultured on plastic and Matrigel. MCF-7 cell lines were reverted to normal phenotype via AIIB2 and LY 294002 inhibitors against beta1 integrin and class I phosphatidylinositol 3-kinase, respectively. MCF-10A acini were distinguishably different from MCF-7 on Matrigel. MCF-10A formed organized hollow spherical structures which were in stark contrast to the MCF-7 disorganized cluster of cells. Matrigel allowed visual monitoring of MCF-7 cells treated with inhibitors. After treatment of MCF-7 cells, we observed reversion of MCF-7 phenotype toward normal, comparable to MCF-10A acini. The 3D culture provides a microenvironment which allows malignant and non-malignant cells to demonstrate near physiological behavior and this can distinguish nonmalignant from malignant cells. The 3D culture also allows visual monitoring of malignant phenotype reversion to organized spheres
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Index:
IMEMR
Main subject:
Phenotype
/
Proteoglycans
/
Breast Neoplasms
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Collagen
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Laminin
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Cell Culture Techniques
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Tissue Culture Techniques
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Drug Combinations
Limits:
Female
/
Humans
Language:
En
Journal:
Iran. Biomed. J.
Year:
2009