Nandrolone decanoate and tamoxifen related histomorphometry: a study in line of osteoporosis

ABSTRACT

To compare the histomorphometric data [in line with osteoporosis] between Tamoxifen treated and Nandrolone decanoate treated female Albino rats through an innovatively modified parameter using SEM [scanning electronic microscopy]. An animal experimental research study was done in which the subjects were ageing six female Albino rats, 8-12 months of age. The animal study was carried out in strict accordance with the guidelines of the Institutional Animals Ethics Committee [IAEC] and all standard dietary protocols were observed. The Albino rats were divided into two main groups comprising of three rats in each group. Group [A] was Tamoxifen treated, 5 mg/kg body weight s/c daily and Group [B] Nandrolone decanoate treated 3 mg/kg body weight, I/M per week. Both groups were treated for six weeks. On completion of treatment the animals were sacrificed, dissected and bones were removed. Quantitative and qualitative data was developed through SEM. The histomorphometric data in this study was Specific Bone Surface [SBS] Specific Eroded Surface [SES] and Total Bone Surface [TBS]/Total Eroded Surface [TES]. Comparison of these parameters was done by the SEM for the two groups of Albino rats i.e. group A; Nandrolone decanoate treated and group B; Tamoxifen treated. The data obtained was evaluated statistically and no significant variation was found between the two groups. The histomorphometric data shows that the effects of Nandrolone decanoate and Tamoxifen in female Albino rats are similar in terms of structural measurement such as SBS, SES and TBS/TES