Expression of cyclin Dl and PI 6 in esophageal squamous cell carcinoma

ABSTRACT

Esophageal squamous cell carcinoma [ESCC] is one of the lethal cancers with a high incidence rate iri Asia. Many genes including cyclin Dl and pi6 play important role in its carcinogenesis. We aimed to analyze the expressions of cyclin Dl and pi6 with the various clinicopathological characteristics of ESCC. We examined 30 biopsy samples of ESCC for cyclin Dl and p!6 protein expressions using immunohistochemistry. Immunointensity was classified as no immunostaining [-], weakly immunostaining [+], weak immunostaining [H-] and strongly positive immunostaining [+++]. Out of the 30 cases, positive expression of cyclin Dl was detected in 26 cases [86.7%]. The percentage of tumors with invasion to the adventitia [88.2%], lymph node metastasis [87.5%], and tumors which were poorly differentiated [92.9%] were higher in cyclin Dl positive tumors than in the cyclin Dl negative tumors. However no significant association was found between cyclin Dl expression and the different clinicopathological parameters.There were 22 cases of ESCC [73.3%] which showed negativity for pi6. The percentage of tumors with invasion to the adventitia [82.4%] and poorly differentiated tumors [92.9%] were higher in the pi6 negative tumors than in the pi6 positive tumors. There was significant association between the histological grade and p16 expression [p=0.012]. However, there were no significant association with regard to site, size and lymph node status of the tumors and p 16 expression