effects of topical application of octy1-2-cyanoacrylate tissue glue on the wond healing in mice: An experimental study

ABSTRACT

Management of patients with full thickness wound of skin continues to challenge physicians and surgeons in area of cosmetic dermatologic surgery. at the present, there are some medications and procedures that can be used to accelerate the healing of full thickness wound of skin. Based on studies that have demonstrated improved upper eyelid wound healing with OCA [octy1-2-cyanoacrylate tiissue glue] treatment, we anticipated that OCA will accelerate healing of full thickness woynd of skin. the aim of research is to evaluate the efficacy of OCA in accelerating healing of full thickness wound of skin in mice. Design: experimental study,, Animal Male N. mRi mice, sample size 6 per study group. Surgery two mm full thickness wound of skin back of mice under general anaestesia. treatment groups Control group simple dressing with sterile guaze, OCA group topical application octy 1-2-cyanoacrylate [Dermabond; ethicon Inc, Somerville, NJ] tissue glue. study Period mice mice were euthanized on days 4, 7 and 10 post-operation to reflect different phases of wound healing, Assay 1- groos pathology of the skin noting presence of infections, dehiscence and repair, 2- histological evaluation of the wound site for the degree of healing, 3- finally the wounds were tested for, Resilience = ability of the wound to stretch and then resume shape without incrring any tissue damage, ultimate strength = maximum pressure a wound can tolerate before it sarts to weaken and toughness = total amount of pressure a wound can tolerate before rupturing. OCA increased 1] formation of granulation tissue 2] density and activation of fibroblasts 3] keratinization in surface of wound 4] thickness of basement membrane and epidermis. OCA decreased toughness of wound in mice. we conclude that OCA accelerates healing of full thickness wound of skin in mice