Generation of mouse spermatogonial stem-cell-colonies in a non-adherent culture

ABSTRACT

Objective: The properties of self-renewal and division in spermatogonial stem cells [SSCs] support spermatogenesis. There is a number of reported methods for in vitro SSC culture systems. The development of a culture system that effectively supports isolation and self-renewal of germline stem cells [GSCs] is of tremendous benefit for clinical trials, experimental research, and as potential treatment for male infertility. The current study aims to consider the cultivation and behavior of GSCs in a non-adherent culture system