Intracellular signaling mechanisms pharmacological action of Jasminum amplexicaule Buch.-Ham. [Oleaceae] on gastrointestinal secretion

ABSTRACT

Jasminum amplexicaule Buch.-Ham. [Oleaceae] has been commonly used in the traditional medicine in dysentery, diarrhoea and bellyache in China. In the present work, the methanol extract of Jasminum amplexicaule [JME] was examined for pharmacology on human colonic epithelial cell line T84 by the short-circuit current technique. The results showed that pretreatment of T84 cells with JME produced a concentration-dependent [0-1000 g/ml. EC50 = 0.055 mg/ml] inhibition effect on adrenalin [Adr.]-induced Cl- secretion. The maximal response was observed at 200 microg/ml. It has been demonstrated that JME has a direct effect on the enterocyte. Our results also demonstrated that the JME exerted inhibitory effect on gastrointestinal Cl- secretion that effected by acting on basolateral beta-adrenoreceptors. These results suggest that the Chinese traditional medicine of JME can be used for the treatment of acute diarrhea and bellyache