Interaction of amantadine and phencyclidine with the nicotinic acetylcholine receptor/channel from torpedo electric organ and philosamia ricini brain

ABSTRACT

In vitro interaction of amantadine and phencyclidine [PCP] with the nicotinic acetylcholine receptor/channel from Philosamia ricini brain and Torpedo electric organ was studied using [3H]PCP as channel probe. The results showed that binding of [3H]PCP to ionic channel of P. ricini brain was potentiated by amantadine, but the binding to ionic channel of Torpedo membranes was inhibited [Ki value is 2 mM]. Amantadine was suggested to act as agonist for ionic channel in insect brain. In addition, Scatchard analysis of the binding indicated at least two sites in P. ricini brain [a high affinity site with kd 5.3 mM and a low affinity site with kd 21.7 nm]. PCP inhibited the binding of [3H]PCP to ionic channel of P. ricini brain and Torpedo membranes with Ki values [0.013-0.016 mM] and 0.8 mM, respectively