Cellulolytic activity of thermomonospora fusca I. selection, characterization and determination of optimal growth conditions for maximal production of cellulase enzyme copmlex
Iraqi Journal of Microbiology. 1999; 11 (2): 71-90
in En
| IMEMR
| ID: emr-50864
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EMRO
ABSTRACT
Thirty one thermophilic, celluloytic actinomycete isolates were selectively isolated from soil, compost, plant-waste biomass and ruminant manure samples, using Kosmachev liquid medium amended with 1% cellulose. Qulitative assessment of celluloytic acitivty was done measuring diameter of the clear zone by the growing bacterial colonies on soild medium containing cellulose. The relatively most active isolates were TA[10], TA[19] and TA[22]; subsequently were characterized and designated as Thermomonospora fusca Optimal growth conditions [time of incubation, temperature and pH] affecting cellulase complex production has been studied. Results showed that, optimal time for maxiarnl enzyme production was [192 216, 144]h. for isolates TA[10] and TA[22] respectively at an optimal temperature of [45°C], and pH values [8,9,9] respectively. Depletion of cellulose as the sole carbon and energy source, and the liberation of reducing sugars, were studied in relation with the cellular growth of the selected isolate TA[19], 810 mg/ml of reducing sugares were accumulated after 216h of incubation, with the concomitant consumption of 82% of cellulose after 240h. of growth
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Index:
IMEMR
Main subject:
Actinobacteria
/
Cellulases
Language:
En
Journal:
Iraqi J. Microbiol.
Year:
1999