Evaluation of direct fluorescent monoclonal antibody test for detection of cryptosporidium in diarrhoeic patients

ABSTRACT

In a trial for evaluation of direct fluorescent monoclonal antibody test [Mab] in diagnosis of cryptosporidiosis, stool of 250 diarrhoeic patients, collected from 150 immunocompetent and 100 immunosuppressed children at different ages, were subjected for both modified Ziehl-Neelsen stain [MZN] and Mab. The obtained data showed that Mab was sensitive, easy and quick to perform in addition to its ability for detection of scanty infection. However, high cost and rapid fading of parasites are its main disadvantages. Although MZN showed less sensitivity to detect Cryptosporidium oocyst, it had the advantage of recognition of other protozoa e.g. G. lamblia. It is concluded that Mab proved to be more superior diagnostic technique, as it could be helpful for epidemiological screening for cryptosporidiosis