Spectrophotometric determination of ampicillin, dicloxacillin, flucloxacillin and amoxicillin in pharmaceutical preparations using potassium periodate

ABSTRACT

Different Spectrophotometric methods were used for the determination of beta-lactam antibiotics using complex formation with Cu [II][1-6] and nickel [II][7]ions or titration against Cu [II] ions using ion-selective electrode [8,9]. Modified Spectrophotometric methods were developed for the determination of some important antibiotics including amoxicillin and ampicillin through charge transfer complexation reaction with chloranil [10,11] Amoxicillin and ampicillin were determined spectrophotometrically in pharmaceutical preparations using potassium iodate [12] picric and picramic acids [13] some nitro compounds [14] certain pi-acceptors[15] or with ammonium molybdite [16]. A Spectrophotometric method based on coupling of amoxicillin with different diazotized primary aromatic amines was developed [17]. Amoxicillin was determined spectrophotometrically in a binary mixture with dicloxacillin[18] or in the presence of dicloxacillin or flucloxacillin[19] Dicloxacillin [in urine] and flucloxacillin [in some pencillin derivatives] were determined using TLC spectrophotometry [20,21]. Two Spectrophotometric methods [22] [using N-bromos- uccinimide and N-chtorosuccinimide] or two colourimetric methods [23] [using sodium hypochlorite and 1-chlorobenzotriazole] were described for the determination of amoxicillin. Ampicillin was determined spectrophotometrically in tablets, capsules, powder and granules with the use of Na 3, 4 -naphthaquinone -1- sulphonate [24]or with l-fluoro-2,4-dinitrobenzene[22] reagents. HPLC methods [26,27] were used for the determination of amoxicillin in different pharmaceutical preparations