Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of strongyloidosis
Pakistan Journal of Medical Sciences. 2004; 20 (1): 36-40
in En
| IMEMR
| ID: emr-68053
Responsible library:
EMRO
ABSTRACT
To evaluate an enzyme- linked immunosorbaent assay using filariform larvae of Strongyloides stercoralis to detect specific antibodies in patients with strongytoidosis. Design:
Serum samples obtained from individuals infected with strongytoidosis and other parasitic diseases as well as normal people were analyzed by IgG-ELISA using crude antigen of filariform larvae.Setting:
School of Public Health Serum Blood Bank, Tehran University of Medical Sciences, volunteers people and selected patients.Subjects:
Serum samples were obtained from 46 individuals infected with strongytoidosis, 37 from normal individuals and 379 cases from others with different parasitic infections. Main outcomemeasures:
The sensitivity, specificity, positive and negative predictive values of the test.Result:
The cut-off point was 0.537. The sensitivity of the test was 93.47%, whereas the specificity was 96.15%. The positive and negative predictive values were 72.88% and 99.25% respectively. Three individuals with hydatidosis, one with ascariasis and 12 with toxocariasis had antibodies that were reactive against larval antigen. ELISA method using filariform larval antigen provides a sensitive and specific diagnostic assay
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Index:
IMEMR
Main subject:
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Antibodies, Helminth
/
Strongyloides stercoralis
/
Antigens, Helminth
Limits:
Humans
Language:
En
Journal:
Pak. J. Med. Sci.
Year:
2004