Nucleotide sequence of cDNA encoding for preprochymosin in native goat [Capra hircus] from Iran

ABSTRACT

Prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. In the present investigation we report sequence of cDNA encoding goat [Capra hircus] preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. As bovine prochymosin, the caprine prochymosin cDNA encodes 365 amino acids with a prosegment of 42 amino acids and the mature goat chymosin begins with glycine. The preprochymosin nucleotide sequence reported in this study differs from other reported goat sequence [AY389343] in three nucleotides, two of which alter the amino acids at positions 19p and 139